Mouse Genetics

The Mouse Genetics Core Facility provides investigators the possibility of generating genetically engineered mice by transgenic and gene targeting methodologies. The core can generate, genetically manipulate, and select mouse embryonic stem (ES) cells from various genetic strains for injection into blastocysts. Alternatively, these genetically modified ES cells can be differentiated in vitro by the core facility into various cell types for investigator research. The core also offers injection of transgenes into fertilized oocytes.

The facility can advise investigators on the generation of transgenic and targeting constructs for simple knock-out/knock-in and for tissue-specific and conditional mutations as well as strategies for screening transfected ES cells and mouse mutants. In addition, the core facility performs rederivation of "dirty" mice by embryo transfer and cryopreservation of mouse strains (embryos).

 

Our Services

The following is a list of services commonly provided by our facility. If you have a specific service request, please fill out this service request form. Completed service request forms and any inquiries about these services or any additional services not listed below should be forwarded to MouseGenetics@NJHealth.org

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Design and Generation of Constructs
Gene Targeting in Mouse ES Cells
ES Cell Screening for Homologous Recombinants
Blastocyst Injection
Pronuclear Injection
Cryopreservation of Mouse Embryos
Cryopreservation of Mouse Sperm
Speed Cryopreservation
Rederivation of Mouse Lines
Rederivation of Mouse Lines by IVF
Chromosome Counting
Mycoplasma Testing