Advanced Diagnostic Laboratories

Collect

Serum from a plain red top is the only acceptable sample type.
Gel barrier tubes are not acceptable.

Specimen Preparation

Allow blood to clot at room temp or 37°C for 20 to 60 minutes. Centrifuge to thoroughly remove cells and immediately transfer cell-free serum to a fresh tube and freeze the cell-free serum on dry ice or at -70°C.
Preferred volume: 1 mL

Pediatric Collection

Minimum volume: 250 µL serum, frozen

Unacceptable Conditions

Thawed specimen.

Storage Transport Temp

Send frozen serum Priority Overnight via FedEx and in a well insulated container on dry ice.

Stability

Frozen at -70°C: 1 year

Methodology

Hemolytic Assay

Reported

up to 4 weeks

Department

Complement Lab

Synonyms

Hemolytic Assay (C4 Function);Complement (C4 Function)

Reference Interval

Human Male: 400,000-43,000,000 Units/mL
Human Female: 400,000-43,000,000 Units/mL

CPT Code

86161