Advanced Diagnostic Laboratories

Glucocorticoid Lymphocyte Stimulation

Test Code

GCLS

Description

Collect

Preferred volume: Three 10 mL Green (Sodium Heparin) top heparinized tubes.

Pediatric Collection

Minimum volume: Two10 mL Green (Sodium Heparin) top heparinized tubes.

Unacceptable Conditions

Centrifuged specimens, specimens received after 24 hours of draw, frozen specimens, specimens in lithium heparin, specimens in tubes with gel such as SST's and PSTs.

Storage Transport Temp

Send ambient blood Priority Overnight via FedEx and in a well insulated container.

Specimen must be received in the laboratory within 24 hours of collection.

Samples will be received Mon - Fri, no later than noon on Friday.

Stability

AMBIENT: 24 HOURS

Notes

Cells are checked for their sensitivity to the suppressive effect of the steroids tested based on the IC50 (measures the amount of a given glucocorticoid to inhibit the activation of lymphocytes by 50%) and Imax (maximum suppression).

A Steroid Kinetics Questionnaire is required prior to completing any of the above tests. You may download and print the questionnaire. Please send completed form along with requisition.

Performed

Mon, Tue and Fri

Methodology

Lymphocyte proliferation

Turnaround Time

7-10 days

Department

Immunology Lab - Functional Assay

Synonyms

lymphocyte proliferation (Glucocorticoid Lymphocyte Stimulation);LPT (Glucocorticoid Lymphocyte Stimulation);steroid insensitivity (Glucocorticoid Lymphocyte Stimulation);steroid resistance (Glucocorticoid Lymphocyte Stimulation)

Study Offerings

RUO
CAP/CLIA

Related Tests

Asthma, Mycoplasma pneumoniae by PCR (MYPCR), Chlamydia pneumoniae by PCR (CHLU), Cortisol pharmacokinetics (CORTH), Beta 2 adrenergic receptor genotype (B2AR), Sptum eosinophils (SSPU), Eosinophil cationic protein (ECP)