Advanced Diagnostic Laboratories

Rifampin Level

Test Code

PKRIF

Description

Rifampin (RIF) is a first-line anti-tuberculosis (TB) and antimicrobial agent used in combination therapy for Mycobacterium tuberculosis infections, nontuberculous mycobacterial (NTM) infections, and certain bacterial infections such as Staphylococcus aureus endocarditis. Due to its complex pharmacokinetics and significant interpatient variability, therapeutic drug monitoring (TDM) is recommended to optimize efficacy while minimizing toxicity and drug resistance.

Collect

Collect Serum in an 8-10 mL plain red top tube. Any other type of tube may influence accurate drug level quantification.

Patient Prep

Allow to clot for 30 minutes, separate serum from cells immediately by centrifugation and aliquot into a labeled polypropylene or similar plastic tube. Use a separate tube for each test ordered. Allow room for expansion of sample inside tube.

Preferred volume: 2 mL of serum (or plasma).
Minimum volume: 0.5 mL serum (or plasma).

Pediatric Collection

If minimum volume cannot be obtained, samples can be ran with dilution calculations. 5x maximum dilution to be allowed.

Unacceptable Conditions

Thawed samples for greater than 6 hours

Storage Transport Temp

Fully complete PK requisition, Missing elements may delay resulting.

Ship samples via overnight delivery with appropriate amount of dry ice to remain frozen upon arrival.

Stability

Freeze at -70C if possible, but at minimum -20C. Up to 6 hours at room temperature.

Notes

Ship samples to be received Monday through Friday. Do not ship on Friday or Saturday.

Performed

Monday - Friday

Methodology

HPLC-UV

Turnaround Time

Within 10 business days. Weekends and holidays may delay results until following business day.

Department

Pharmacokinetics Lab

Synonyms

Rifadin (Rifampin, Level), Mycobacteria (Rifampin, Level), Rimactane (Rifampin, Level), HPLC (Rifampin, Level), pharmacokinetics (Rifampin, Level), RIFH

Study Offerings

RUO
CAP/CLIA