Advanced Diagnostic Laboratories

Collect

Collect in a plain red top or SST tube

Specimen Preparation

Allow sample to clot at room temperature for 20 to 60 minutes. Centrifuge ASAP to separate serum from cells and aliquot cell-free serum into a labeled polypropylene or similar plastic tube.

Preferred volume: 0.5 mL serum
 

Pediatric Collection

Pediatric Collection
Minimum volume: 0.1 mL
 

Storage Transport Temp

Send serum Priority Overnight via FedEx and in a well-insulated container frozen on dry ice.
 

Stability

Frozen at -70°C: 1 year. Serum samples may be stored at +2°C to +8°C for up to 14 days if necessary.
 

Notes

National Jewish Health Advanced Diagnostic Laboratories offers a Scleroderma (systemic sclerosis) Panel to aid in the diagnosis of systemic sclerosis. When combined with clinical features, the Scleroderma Antigen Panel can aid clinicians in making a diagnosis of systemic sclerosis and related conditions. The Scleroderma Antigen Panel provides a qualitative in vitro assay for human antibodies present in serum of the IgG class to 12 different antigens.
 

Performed

Weekly
 

Methodology

Immunoblot
 

Reported

2-12 days
 

Department

Immunology Lab - Diagnostic

Synonyms

Scl-70, CENP A, centromere A, CENP B, centromere B, RP11, RP155, Fibrillarin, NOR90, Th/To, PM/Scl-100, PM/Scl-75, Ku, Ro-52

Reference Interval

Negative 0-5 Units
Borderline 6-10 Units
Positive 11-50 Units
Strong Positive >50 Units
 

Interpretation

Antibodies to the antigens are determined by an immunoblot assay. The color developed for each antigen is determined by densitometry and the intensity of the color is proportional to the antibody reactivity. The results are given in arbitrary intensity units.

CPT Code

84182