Advanced Diagnostic Laboratories

Collect

Draw one green top tube (Sodium or Lithium Heparin) top tube.

Specimen Preparation

Do NOT centrifuge! Keep at room temperature (18-22°C).

Preferred volume: 4mL

Pediatric Collection

Minimum volume: 1mL

Unacceptable Conditions

Frozen, clotted, hemolyzed or centrifuged specimens. Anticoagulants other than heparin. Specimens >24 hrs old.

Storage Transport Temp

Maintain and transport specimens at room temperature (18-22°C).
Ship specimens Priority Overnight via Fed Ex in container sufficiently insulated to avoid temperature extremes (<10°C and >37°C).

Stability

24 hours at room temperature (18-22°C)

Notes

Samples must arrive within 24 hours of draw and are accepted Monday through noon on Friday.

Performed

Tue-Fri

Methodology

Flow Cytometry

Reported

2-4 days

Department

Immunology Lab - Phenotyping Assays

Synonyms

Autoimmune lymphoproliferative syndrome;Double negative T cells

Reference Interval

<2.5% Of T Cells

Interpretation

This test is useful for assessing the percentage of CD4-CD8- (double negative) T cells or DNTs. DNT > 1% supports the diagnosis of autoimmune lymphoproliferative syndrome (ALPS).

CPT Code

88185, 88184 x 3