Neutrophil Adherence Markers (includes CD11a, CD11b, CD11c and CD18)
Test Code
ADHM
Description
Collect
Draw one green top tube (Sodium or Lithium Heparin) top tube.
Pediatric Collection
Minimum volume: 2 mL blood
Unacceptable Conditions
Frozen, clotted, hemolyzed or centrifuged specimens. Anticoagulants other than heparin. Specimens >24 hrs old.
Storage Transport Temp
Maintain and transport specimens at room temperature (18-22°C).
Ship specimens Priority Overnight via Fed Ex in container sufficiently insulated to avoid temperature extremes (<10°C and >37°C).
Stability
24 hours at room temperature (18-22°C)
Notes
Samples should be received within 24 hours of draw. Samples are accepted Monday through noon on Friday.
Reference Interval
- CD11a: >29 Mean Fluorescence Intensity (MFI)
- CD11b: >154 MFI, CD11c: >113 MFI, CD18: >192 MFI
Interpretation
LAD-1 is an autosomal recessive disorder caused by mutations in CD18, the common chain of the beta-2 integrin family. The beta-2 integrin family is comprised of LFA-1 (CD11a/CD18), Mac-1/CR3 (CD11b/CD18) and p150/95 (CD11c/CD18). Defects in CD18 therefore affect the expression of CD11a, b and c. Reduction or loss of surface expression of the beta-2 integrins supports the diagnosis of LAD-1.
CPT Code
88184, 88185 X 3
New York Approved
No: Run with Waiver
FDA
LDT